Comparative studies on the composition of 15 phenotypes of Drosophila melanogaster Mg (adults and larvas)

Abstract

Comparatlve Studies on the Composition of 15 Phenotypes of Drosophila melanogaster Mg (adults and larvas). Rev. esp. Fisiol.. 30, 289-298. 1974.

Relatively little Information is available on the Chemical composition of some class of phenotypes of Drosophila melanogaster Mg. This work has been carried out in order to compare the composition (humidity, proteins, lipids and carbohydrates) of the following 15 class of phenotypes (corresponding to their homozygous genotypes) of D. melanogaster: «Silvestre» (+ Madrid), «brown», «cinnabar», «purple», «sepia», «scarlet», «white», «white apricot», «black», «ebony», «yeliow», *»«,yellow «dumpy», «vestigial» and «Curly».

The determinations were made in adults and in larvas. Valúes obtained (as % of dry weight) were: For adults (for larvas, between brackets): Humidity, 68-74 % (71-81 %). Total protein, 34-44 % (25-35 %); a máximum of 8 bands were separated by disc electrophoresis of polyacrylamide gel, with a molecular weight between 32,000 and 250,000. Total lipids, generally 20-34 % (near 50 %); phospholipids, as % of total lipids, 18-29% (3.3-11.7%); cholesterol, as % of total lipids, 1.1-2.9 % (0.8-1.5 %). Glucose > mannose > galactose > ribose were found in all phenotypes, but fucose in some of them only; galactosamine and glucosamine were also identified, and determined in higher amounts in adults than in larvas. No sialic acids were detected.