Quantitative analysis of steroids in urine by gas-liquid chromatography

J.M. Macarulla
J.A. Gómez-Capilla
C. Osorio
55

Abstract




Ouantitative Analysis of Steroids ¡n Uriníƒe by Gas-liquid Chromatography. Rev. esp. FisioL, 30, 67-70. 1974. A method of acid hydrolysis of steroid substances in uriníƒe, with simultaneous extrac- tion with trichloretylene as organic solvent, is developed. The qualities of this solvent


for the extraction of steroids from aqueous solution are discussed.
Doing, in the extract, a direct gas-chromatography, without sylanization of steroid substances, at a programmed temperature from 190° C to 265° C increasing 2.5° C per minute, in 30 minutes, it is possible to obtain a quantitative analysis of 17-ketosteroids, testosterone, pregnandiol, allo-pregnandiol and cholesterol, using cholestanone as inter­ nal standard.


The method seems adequate for the clinical follow up of pregnancy and also for diagnosis and clinical follow up of adrenal cortex disfunction.




Keywords:
Chromatography, 17-Ketosteroids/urine, Adrenocortical Hyperfunction/urine, Androstenedione/analogs and derivatives/urine, Androstenols/urine, Androsterone/urine, Cholestanes/urine, Cholesterol/urine, Dehydroepiandrosterone/urine, Etiocholanolone/urine, Female, Gas/methods, Humans, Pregnancy, Pregnanediol/urine, Pregnanetriol/urine, Solvents, Steroids/urine, Testosterone/urine, Time Factors, Trichloroethylene

Authors

J.M. Macarulla
J.A. Gómez-Capilla
C. Osorio


Metrics

Search GoogleScholar



Downloads

Download data is not yet available.

Section

Articles

Most read articles by the same author(s)

<< < 1 2 3 4 5 6 7 > >>