Quantitative analysis of steroids in urine by gas-liquid chromatography

Abstract

Ouantitative Analysis of Steroids ¡n Uriníƒe by Gas-liquid Chromatography. Rev. esp. FisioL, 30, 67-70. 1974. A method of acid hydrolysis of steroid substances in uriníƒe, with simultaneous extrac- tion with trichloretylene as organic solvent, is developed. The qualities of this solvent

for the extraction of steroids from aqueous solution are discussed.
Doing, in the extract, a direct gas-chromatography, without sylanization of steroid substances, at a programmed temperature from 190° C to 265° C increasing 2.5° C per minute, in 30 minutes, it is possible to obtain a quantitative analysis of 17-ketosteroids, testosterone, pregnandiol, allo-pregnandiol and cholesterol, using cholestanone as inter­ nal standard.

The method seems adequate for the clinical follow up of pregnancy and also for diagnosis and clinical follow up of adrenal cortex disfunction.