Free endogenous aminoacids in perfused intestine in rats

Abstract

The intestinal content of free aminoacids from endogenous origin is studied by perfusion through the small intestine of rats under the influence of several factors, pancreatic and biliary secretions, the addition of L-glutamic acid at two different concentrations to the perfusion liquid either with the pancreatic and biliary secretion or without them. The conclusion is reached that the pancreatic juice contributes significantly to the intestinal amount of free aminoacids; the addition of L-glutamic acid to the perfusion liquid under the fixed experimental conditions decreases the amount of free amino acids transfered by active mechanisms in the intestinal contents. This effect shows a certain selective preference to the essential aminoacids against the non-essential aminoacids. Such effect is not observed, however when the pancreatic secretion is not present in the intestinal contents.