Determination of lecithin: cholesterol acyltransferase activity by a radioisotopic assay

M.R. Ras; S. Masdeu; C. Miquel; J.C. Frisón; J. Rubiés-Prat; S. Schwartz

M.R. Ras

S. Masdeu

C. Miquel

J.C. Frisón

J. Rubiés-Prat

S. Schwartz

PDF (Spanish) (

23)

Published: 1976-06-01

Keywords:

Acyltransferases/blood, Carbon Radioisotopes, Cholesterol/blood, Esterification, Humans, Methods, Phosphatidylcholine-Sterol O-Acyltransferase/blood

How to Cite

Determination of lecithin: cholesterol acyltransferase activity by a radioisotopic assay. (1976). Revista Española De Fisiología, 32(2), 95-98. https://revistas.unav.edu/index.php/ref/article/view/49502

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Abstract

A method for determining Lecithin: Cholesterol Acyltransferase (LCAT) activity is presented. Trace amounts of labelled cholesterol are added to innactivated homologous substrate from a pool of normal serum. LCAT activity is determined measuring the cholesterol esterification, and is expressed as micrograms of cholesterol esters formed per milliliter plasma per hour. LCAT time-course, reproducibility, activity after serum storage at 4 degrees C, and normal values from 20 healthy subjects are studied.

Keywords:

Acyltransferases/blood, Carbon Radioisotopes, Cholesterol/blood, Esterification, Humans, Methods, Phosphatidylcholine-Sterol O-Acyltransferase/blood

Authors

M.R. Ras

Dr. M. R. Ras, Servicio de Bioquímica, Ciudad Sanitaria de la Seguridad Social, Avda. Valle Hebrón, s/n, Barcelona - 16 (España)

https://orcid.org/0000-0002-1825-0097

S. Masdeu