The determination of urinary pregnandiol by thin-layer chromatography, with quantitative densitometry: comparative study with a spectrophotometric method
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26)
Published:
1972-06-01
Keywords:
Chromatography, Densitometry, Spectrophotometry, Evaluation Studies as Topic, Humans, Pregnanediol/urine, Thin Layer
How to Cite
The determination of urinary pregnandiol by thin-layer chromatography, with quantitative densitometry: comparative study with a spectrophotometric method. (1972). Revista Española De Fisiología, 28(2), 129-134. https://revistas.unav.edu/index.php/ref/article/view/49319
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Abstract
A method for the quantitative determination of urinary pregnandiol is presented, being based on the application of photodensitometry to thin-layer chromatoplates, revealing the steroid spots by means of an unspecific reagent.
A comparison is established with the spectrophotometric method of Klopper et al., obtaining a close correlation in values higher than 1.5 mg/1000 ml. The reasons for the deficient correlation obtained in concentrations lower than the above mentioned one ase discussed in this work.
The possible advantages it can offer in its application to routinary laboratories are also pointed out.
Keywords:
Chromatography, Densitometry, Spectrophotometry, Evaluation Studies as Topic, Humans, Pregnanediol/urine, Thin Layer
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