Effect of synthetic LH-RH and hypothalamic extracts on RNA synthesis by rat pituitaries in vitro

Abstract

Incorporation of 14C-Uridine in pituitary RNA from adults or prepuberal female rats in vitro, incubated with synthetic LH-RH or hypothalamic extracts, was studied in this work. RNA synthesis was not increased by any of the assayed stimuli in adult female rat pituitaries. The 14C-Uridine incorporation in pituitary prepuberal female rats increased in presence of synthetic LH-RH or adult female hypothalamic extracts, but not in presence of prepuberal female hypothalamic extracts. The differences found in the in vitro behaviour between pituitaries from prepuberal and adult female rats, and between their respective hypothalamic extracts, are attributables to the evolution of these organs along the sexual maturing process.