Assay of mitochondrial membrane-bound enzyme activities in the presence of triton X-100

J.I. Gurtubay
J. Martínez
A. Gutiérrez-Arranz
F.M. Goñi
50

Abstract

Mitochondrial ATPase and cytochrome c oxidase activities are not severely affected by Triton X-100 concentrations between 0.1 and 2.0% (w/v). The former is solubilized by the effect of the detergent, while the latter is not. Succinate: cytochrome c reductase and rotenone-sensitive NADH: cytochrome c reductase activities are destroyed even a low detergent concentrations. Succinate:coenzyme Q oxidoreductase is affected by the surfactant in a more complex way, so that selective solubilization of some subunit(s) could be involved.

Keywords:
Adenosine Triphosphatases/metabolism, Animals, Electron Transport Complex IV/metabolism, Enzyme Activation/drug effects, Enzymes/metabolism, Liver/enzymology/metabolism, Male, Membranes/metabolism, Mitochondria, NADH Dehydrogenase/metabolism, Polyethylene Glycols/pharmacology, Rats, Succinate Cytochrome c Oxidoreductase/metabolism

Authors

J.I. Gurtubay
J. Martínez
A. Gutiérrez-Arranz
F.M. Goñi


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