Influence of cephalosporins on intestinal enzymatic activity
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Abstract
The effect of the broad spectrum antibiotics cefaclor, cefadroxil, cephradine, cefatrizine, ccphaloglycine and cefroxadine was examined on rat intestinal brush border enzymes, aminopeptidase N (E.C. 3.4.11.2), dipeptidyl peptidase IV (E.C. 3.4.14.5) and alkaline phosphatase (E.C. 1.3.1.3.). All the cephalosporins assayed -except cefaclor-inhibit the aminopeptidase N activity, in an uncompetitive manner. Cefatrizine showed the most important inhibitory effect (52.5 %; p < 0.001). Cefaclor and
cefadroxil have no effect on the activity of the dipeptidyl peptidase IV, while ccphaloglycine and cephradine showed a non competitive type inhibition. In contrast, cefatrizine and cefroxadine showed a competitive inhibition for this enzyme.
None of the cephalosporins assayed had any effect on alkaline phosphatase activity.
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