Influence of cephalosporins on intestinal enzymatic activity
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Abstract
The effect of the broad spectrum antibiotics cefaclor, cefadroxil, cephradine, cefatrizine, ccphaloglycine and cefroxadine was examined on rat intestinal brush border enzymes, aminopeptidase N (E.C. 3.4.11.2), dipeptidyl peptidase IV (E.C. 3.4.14.5) and alkaline phosphatase (E.C. 1.3.1.3.). All the cephalosporins assayed -except cefaclor-inhibit the aminopeptidase N activity, in an uncompetitive manner. Cefatrizine showed the most important inhibitory effect (52.5 %; p < 0.001). Cefaclor and
cefadroxil have no effect on the activity of the dipeptidyl peptidase IV, while ccphaloglycine and cephradine showed a non competitive type inhibition. In contrast, cefatrizine and cefroxadine showed a competitive inhibition for this enzyme.
None of the cephalosporins assayed had any effect on alkaline phosphatase activity.
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References
Alcalde, A. I., Barcina, Y" Ilundain, A. and Larralde, J. (1987): Drug-Nittr. Interact., 4, 71-79 .
Auricchio, S. (1983): J. Pediatr Gastroent. Nt
Barcina, Y., Ilundain, A. and Larralde, J. (1988): Dri
Bradford, B. B. (1976): Anal. Biochem., 72, 248- 254 . https://doi.org/10.1006/abio.1976.9999
Cooke, D. M., Salter, A. J. and Philips, I. (1993): J. Antimicrob. Chemother., 11, 447-453 6. Dahlqvist, A. (1984): Anal. Biochem., 7, 18-25 . https://doi.org/10.1093/jac/11.5.447
Dupuis, Y., Tardivcl, S., Porembska, Z. and Fournier, P. (1991): hit. J. Biochem., 23, 175- 180 . https://doi.org/10.1016/0020-711X(91)90186-Q
Fairclough, P. and Beechey, R. B. (1990): Gastroenterology, 98, 676-685 . https://doi.org/10.1016/0016-5085(90)90288-C
Gilles-Bailiien, M. (1984): Mol. Physiol., 6, 365- 376 .
Hong, W. and Dovle, D. (1990): J. Cell Biol., 111,323-328 . https://doi.org/10.1083/jcb.111.2.323
Kameda, Y., Asano, N., Takeuchi, M., Yamaguchi, T., Matsui, K., Horii, S. and Fukase, H. (1985):/. Antibiot., 38, 121816-121818 . https://doi.org/10.7164/antibiotics.38.1816
Kramer, W., Dechent, C., Girbig, F., Gutjahr, U. and Neubauer, H. (1990): Biochim. Biophys. Acta, 1030, 41-49 . https://doi.org/10.1016/0005-2736(90)90236-H
Leng-Peschlow, E. (1989): Digestion, 44, 200- 210 . https://doi.org/10.1159/000199912
Martin, J. C. and Evans, W. J. (1991): J. Inorg .Biochem., 42, 161-175 . https://doi.org/10.1016/0162-0134(91)84002-Q
Mendizabal, M. V., Idoate, I., Jordan, J. and Larralde, J. (1991): Arch. Int. Physiol. Biochim Biophys., 99,317-320 . https://doi.org/10.3109/13813459109146930
Mendizabal, M. V., Idoate, I. and Larralde, J (1990): Comp. Biochem. Physiol., 96C, 247-250 . https://doi.org/10.3109/13813459109146930
Murer, H. and Hildman, B. (1984): In "Pharmacology of intestinal permeation". Springer-Verlag New York., pp. 157- 193 . https://doi.org/10.1007/978-3-642-69505-6_6
Nicholson, J. A., McCarthy, D. M. and Kim, Y .S. (1980): J. Clin. Invest., 54, 890-898 . https://doi.org/10.1172/JCI107828
Raul, F., Goda, T., Gosse, F. and Koldovsky, O. (1987): Biochem. J., 247, 401-405 . https://doi.org/10.1042/bj2470401
Rollin, J. A. (1986): Am. J. Vet. Res., 47, 987- 991 . https://doi.org/10.1016/0022-3697(86)90113-7
Sahara, N., Fukasawa, K., Harada, M. and Suzuki, K. (1989): Acta Histochem. Cytochem., 22, 479-486 . https://doi.org/10.1267/ahc.22.479
Seetharam, B., Yeh, K. Y. and Alpers, D. H. (1980): Am. J. Physiol., 239, G524-G531 . https://doi.org/10.1152/ajpgi.1980.239.6.G524
Scmenza, G. (1986): Ann. Rev. Cell. Biol., 2, 255-313 . https://doi.org/10.1146/annurev.cb.02.110186.001351
Shirazi-Beechey, S. P., Davies, A. G., Tcbbutt, K., Dyer, J., Ellis, A., Taylor, C. J., Smithson, K. W. and Fairclough, R. B. (1990): Gastroenterology, 98, 676-685 . https://doi.org/10.1016/0016-5085(90)90288-C
Sonoyamak, S,. Kirayama, S. and Niki, R. (1994): J. Nntr. Biochem., 5, 291-297 . https://doi.org/10.1016/0955-2863(94)90034-5
Surawicz, C. M., Elmer, G. M., Speelman, P., McFarland, L. V., Chinn, J. and Van Belle, G. (1989): Gastroenterology, 96, 981-988 . https://doi.org/10.1016/0016-5085(89)91613-2
Van Os, G. and Ghinjsen, M. (1983); In "Intestinal transport" (Gilles-Bailiien, M. and Gilles, R., eds.). Springer-Verlag. Heidelberg, pp. 170- 183 .
Wilkes, S. H. and Prescott, J. M. (1985): J. Biol. Chem., 260, 13154-13162 . https://doi.org/10.1016/S0021-9258(17)38851-8
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