Soluble and membrane-bound leucyl- and arginyl-aminopeptidase activities in subcellular fractions of young and adult rat brains

M. Ramírez
G. Arechaga
S. García
B. Sánchez
P. Lardelli
J.M. Gandarias
68

Abstract

The subcellular distribution of soluble and membrane-bound leucyl- and arginyl-aminopeptidase activities were analyzed in one and five month old rat brains using Leu- and Arg-2-naphthylamide as substrates. Both soluble leucyl- and arginyl-aminopeptidase activities showed the highest levels in the synaptosomal fraction in the two groups of rats. The highest levels of membrane-bound leucyl- and arginyl-aminopeptidase activities were found in the microsomal fraction in the two ages studied. There were no differences between the two ages in soluble leucyl- and arginyl-aminopeptidase activities. However, a significant decrease in both membrane-bound enzymatic activities was evidenced in the synaptosomal fraction of older rats. Developmental changes of these aminopeptidase activities in a determined subcellular localization, may reflect modifications in their effect on neuropeptides susceptible to be hydrolyzed in this particular location.

Keywords:
Aminopeptidases/metabolism, Animals, Brain/enzymology, In Vitro Techniques, Inbred Strains, Intracellular Membranes/enzymology, Leucyl Aminopeptidase/metabolism, Male, Rats, Subcellular Fractions/enzymology, Synaptosomes/enzymology

Authors

M. Ramírez
G. Arechaga
S. García
B. Sánchez
P. Lardelli
J.M. Gandarias


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