A simple procedure for direct corticosterone radioimmunoassay in the rat

Abstract

A simple procedure for the radioimmunoassay of corticosterone using tritiated corticosterone has been described for rat.The use of trypsin to hydrolyze serum proteins allowed to eliminate the extraction of the steroid from the serum.A highly significant correlation between direct and conventional (ether extraction) radioimmunoassays was found.Furthermore, the paired "t" test indicated no significant differences between the values obtained with the two methods.