Enzymatic assays: optimization of systems by using pyruvate kinase and lactate dehydrogenase as auxiliary enzymes
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39)
Published:
1982-09-01
Keywords:
L-Lactate Dehydrogenase, Pyruvate Kinase, Animals, Cattle, Cost-Benefit Analysis, Kinetics, Mathematics, Methods, Mitochondria/enzymology, Oxidative Phosphorylation Coupling Factors/analysis, Proton-Translocating ATPases/analysis
How to Cite
Enzymatic assays: optimization of systems by using pyruvate kinase and lactate dehydrogenase as auxiliary enzymes. (1982). Revista Española De Fisiología, 38(3), 327-332. https://revistas.unav.edu/index.php/ref/article/view/46603
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Abstract
A method to optimize enzymatic assays by using pyruvate kinase and lactate dehydrogenase enzymes is presented and applied to mitochondrial ATPase as an example.Optimum amounts of auxiliary enzymes, to obtain either a 99% of the initial rate in a given time (t99) or a given lag period (L), are calculated from their apparent Michaelis constants (Kapp) in the medium used and their prices per enzymatic international unit.
Keywords:
L-Lactate Dehydrogenase, Pyruvate Kinase, Animals, Cattle, Cost-Benefit Analysis, Kinetics, Mathematics, Methods, Mitochondria/enzymology, Oxidative Phosphorylation Coupling Factors/analysis, Proton-Translocating ATPases/analysis
Authors
F.F. Belda
F.G. Carmona
F.G. Cánovas
J.C. Gómez
J.A. Lozano
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