Protein synthesis by chloroplasts in vitro using light as a source of energy

D.E. Griffiths; J.A. Lozano

D.E. Griffiths

J.A. Lozano

PDF (Spanish) (

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Published: 1970-03-01

Keywords:

Photosynthesis, Plant Proteins, Adenosine Triphosphate/metabolism, Amino Acids/metabolism, Chloroplasts/metabolism, Guanine Nucleotides/metabolism, Mercaptoethanol/metabolism, Phosphates/metabolism, Potassium/metabolism, Sodium/metabolism, Vegetables

How to Cite

Protein synthesis by chloroplasts in vitro using light as a source of energy. (1970). Revista Española De Fisiología, 26(1), 83-88. https://revistas.unav.edu/index.php/ref/article/view/49201

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Abstract

Light can be used as a source of energy in the protein synthesis carried out by isolated chloroplasts from young pea leaves. The light dependent incorporation requires photophos- phorylation cofactors: ADP, Pi, PMS, isoascorbate.

The optimum concentrations of Mg+2 (12 mM), K+ (150 mM), mercaptoethanol (16 mM), C12-aminoacids mixture (7 /zg/ml of each one), were similar to the optimum concentrations in the dark incorporation using an ATP-generating system. Chloramphenicol and Ribonu- clease inhibitions were also similar in both systems.

Broken chloroplasts do not incorpórate significantly C14-leucine into protein.

Keywords:

Photosynthesis, Plant Proteins, Adenosine Triphosphate/metabolism, Amino Acids/metabolism, Chloroplasts/metabolism, Guanine Nucleotides/metabolism, Mercaptoethanol/metabolism, Phosphates/metabolism, Potassium/metabolism, Sodium/metabolism, Vegetables