Enzymatic assays: optimization of systems by using pyruvate kinase and lactate dehydrogenase as auxiliary enzymes

F.F. Belda; F.G. Carmona; F.G. Cánovas; J.C. Gómez; J.A. Lozano

F.F. Belda

F.G. Carmona

F.G. Cánovas

J.C. Gómez

J.A. Lozano

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39)

Published: 1982-09-01

Keywords:

L-Lactate Dehydrogenase, Pyruvate Kinase, Animals, Cattle, Cost-Benefit Analysis, Kinetics, Mathematics, Methods, Mitochondria/enzymology, Oxidative Phosphorylation Coupling Factors/analysis, Proton-Translocating ATPases/analysis

How to Cite

Enzymatic assays: optimization of systems by using pyruvate kinase and lactate dehydrogenase as auxiliary enzymes. (1982). Revista Española De Fisiología, 38(3), 327-332. https://revistas.unav.edu/index.php/ref/article/view/46603

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Abstract

A method to optimize enzymatic assays by using pyruvate kinase and lactate dehydrogenase enzymes is presented and applied to mitochondrial ATPase as an example.Optimum amounts of auxiliary enzymes, to obtain either a 99% of the initial rate in a given time (t99) or a given lag period (L), are calculated from their apparent Michaelis constants (Kapp) in the medium used and their prices per enzymatic international unit.

Keywords:

L-Lactate Dehydrogenase, Pyruvate Kinase, Animals, Cattle, Cost-Benefit Analysis, Kinetics, Mathematics, Methods, Mitochondria/enzymology, Oxidative Phosphorylation Coupling Factors/analysis, Proton-Translocating ATPases/analysis