Protein distribution is submitochondrial particles after lipid peroxidation

J. Eugui
C. Gil
J.C. Gómez
J. Segovia
46

Abstract

Three fractions of submitochondrial particles were isolated by differential centrifugation of mitochondrial inner membranes incubated with 1 mM ascorbate. Proteins were separated by polyacrylamide gel electrophoresis and their pattern was found to be different for the three fractions. These results are in agreement with previous work which showed the dissimilar enzyme distribution and chemical composition of these submitochondrial particles and pointed out the heterogeneity of the inner mitochondrial membrane.

Keywords:
Animals, Electrophoresis, Lipid Metabolism, Liver/metabolism, Male, Membrane Proteins/isolation and purification/metabolism, Mitochondria, Oxygen Consumption, Polyacrylamide Gel, Proteins/isolation and purification/metabolism, Rats

Authors

J. Eugui
C. Gil
J.C. Gómez
J. Segovia


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