Effect of calcium on histamine release from pleural and peritoneal mast cells induced by catechol

L.M. Botana
N. Eleno
J. Espinosa
M.P. Fernández-Otero
52

Abstract

Histamine release from rat pleural and peritoneal mast cells induced by catechol (1, 10, 50, 250 microM and 1 mM) has been studied.The dose-response induced by catechol is non-cytotoxic, is not modified by purification of mast cells and is calcium independent.The sensitivity and maximum response to catechol is the same irrespective of the presence or absence of Ca++, except on purified pleural mast cells, that showed a plateau response at 250 microM catechol in the absence of Ca++, and on unpurified peritoneal mast cells which exhibited a lower maximum response equally in the absence of Ca++.The release is induced by catechol at concentrations as low as 50 microM in all cases, and the maximum response is reached at 1 mM.

Keywords:
Animals, Calcium/pharmacology, Catechols, Female, Histamine Release/drug effects, Inbred Strains, Male, Mast Cells/drug effects/metabolism, Peritoneal Cavity/cytology, Pleura/cytology, Rats

Authors

L.M. Botana
N. Eleno
J. Espinosa
M.P. Fernández-Otero


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