Radioimmunoassay of plasma ferritin of Gallus domesticus L

Abstract

A new experimental procedure for chicken plasma ferritin based on the method of Luxton et al.has been carried out.All biologic reagents used were obtained in our laboratory: Ferritin from chicken liver, antiferritin antiserum from rabbits and anti-rabbit IgG from goat.Chloramine T procedure was applied to label ferritin with 125I.The iodinated protein was removed from free iodide by use of three chromatographic columns of Sephadex G-25, Sephadex G-200 and Sepharose 6B.Separation of the first antigen-antibody complex was carried out by the double antibody method.The assay working range covers from 21.8 up to 175 ng/ml.As ferritin concentration is higher in chicken plasma than in human, various plasma dilutions ranging from 1/16 to 1/64 were used.