Isolation and purification of duck liver ferritin

J.M. Díez
M.T. Agapito
J.M. Recio
65

Abstract

A rapid method of purifying duck liver ferritin using high speed centrifugation and chromatography on Sephadex G-200 and Sepharose 6B is described.Protein and iron concentration for each step of purification is given.This method yields 0.12 mg of pure ferritin per gram of wet tissue.

Keywords:
Animals, Chromatography, Ducks/metabolism, Electrophoresis, Ferritins/isolation and purification, Gel, Liver/analysis, Molecular Weight, Polyacrylamide Gel, Ultracentrifugation

Authors

J.M. Díez
M.T. Agapito
J.M. Recio


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