A radioimmunoassay for duck serum ferritin

J.M. Díez; M.T. Agapito; J.M. Recio

J.M. Díez

M.T. Agapito

J.M. Recio

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Published: 1985-12-01

Keywords:

Animals, Chromatography, Ducks/blood, Ferritins/blood/immunology, Gel, Radioimmunoassay/methods

How to Cite

A radioimmunoassay for duck serum ferritin. (1985). Revista Española De Fisiología, 41(4), 457-462. https://revistas.unav.edu/index.php/ref/article/view/46758

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Abstract

Development and optimization of a radioimmunoassay for duck serum ferritin described.125I-labelled ferritin and rabbit anti-ferritin antibody are used together with goat anti-rabbit gamma globulin as separating agent for the bound and free fractions.The assay has a working range of up to 500 micrograms of ferritin per litre and a sample requirement of 50 microliter of serum.The assay requires a 24 h period and has a sensitivity of 10 micrograms of ferritin per litre.

Keywords:

Animals, Chromatography, Ducks/blood, Ferritins/blood/immunology, Gel, Radioimmunoassay/methods