Na+ K+ ATPase activity in mast cells from the pleural and peritoneal cavities of the rat

N. Eleno
L.M. Botana
J. Espinosa
C. Segura
C. Taboada
P. Fernández-Otero
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Abstract

ATPase activity in rat mast cells was studied, assuming that pleural and peritoneal mast cells are different populations.Mast cells were purified with Percoll.Enzymatic activity was found to be 36% higher in pleural cells that in peritoneal cells.Moreover, for both populations results show a Na+-K+ ATPase activity either low or slightly inhibited by ouabain.

Keywords:
Animals, Inbred Strains, Mast Cells/enzymology, Ouabain/pharmacology, Peritoneal Cavity/cytology, Pleura/cytology, Potassium/pharmacology, Rats, Sodium-Potassium-Exchanging ATPase/analysis/antagonists and inhibitors

Authors

N. Eleno
L.M. Botana
J. Espinosa
C. Segura
C. Taboada
P. Fernández-Otero


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