Effect of sulfhydryl reagents on mevalonate kinase partially purified from chicken liver

J. García-Martínez
A. Linares
M.D. Suárez
E. García-Peregrín
53

Abstract

Unlike other vertebrate mevalonate kinase, the enzyme partially purified from neonatal chick liver was not activated by the -SH group protectors reduced glutathione, cysteine, dithiothreitol and beta-mercaptoethanol at any concentrations assayed (0.01-10.00 mM).However, the activity was found to be sensitive to thiol group binding reagents.p-Hydroxymercuribenzoate was the most active inhibitor.At 0.1 mM concentration, p-HMB completely abolished the enzyme activity.N-ethylmaleimide (0.01-1.00 mM) was practically ineffective.Inhibition by p-HMB was temperature dependent, being more potent at 37 degrees C than at 4 degrees C.

Keywords:
Phosphotransferases (Alcohol Group Acceptor), Animals, Chickens/metabolism, Ethylmaleimide/pharmacology, Hydroxymercuribenzoates/pharmacology, Liver/enzymology, Phosphotransferases/antagonists and inhibitors/isolation and purification, Species Specificity, Sulfhydryl Compounds/pharmacology, Sulfhydryl Reagents/pharmacology, Temperature

Authors

J. García-Martínez
A. Linares
M.D. Suárez
E. García-Peregrín


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