Peroxisomal and mitochondrial citrate synthase in CAM plants

M.F. Zafra
J.L. Segovia
M.J. Alejandre
E. García-Peregrín
64

Abstract

Citrate synthase wa studied for the first time in peroxisomes and mitochondria of crassulacean acid metabolism plants.Cellular organelles were isolated from Agave americana leaves by sucrose density gradient centrifugation and characterized by the use of catalase and cytochrome oxidase as marker enzymes, respectively.48,000 X g centrifugation caused the breakdown of the cellular organelles.The presence of a glyoxylate cycle enzyme (citrate synthase) and a glycollate pathway enzyme (catalase) in the same organelles, besides the absence of another glyoxalate cycle enzyme (malate synthase) is reported for the first time, suggesting that peroxisomal and glyoxysomal proteins are synthesized at the same time and housed in he same organelle.

Keywords:
Catalase/isolation and purification, Cell Fractionation, Centrifugation, Citrate (si)-Synthase/isolation and purification, Density Gradient, Malate Synthase/isolation and purification, Microbodies/enzymology, Mitochondria/enzymology, Organoids/enzymology, Oxo-Acid-Lyases/isolation and purification, Plants/enzymology

Authors

M.F. Zafra
J.L. Segovia
M.J. Alejandre
E. García-Peregrín


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