Inhibition of the active transport of sugars by the female rat intestine in vitro. II. Action of atebrin, atractyloside and selenite

Abstract

It is shown that atebrine, atractyloside and selenite, which had been considered as inhibitors of the intestinal absorption of glucose, are inhibitors of the active transport of sugars in sacs of everted jejunum of the rat (Wilson and Wiscman method). Atebrine 5 x 10-3 M and io-a M inhibit, by 85 % and 100 % respectively, the active transport of galactose. With atractyloside io-3 M and 10-3 M, the inhibitions were 77 % and 100 % and, with selenite io-4 M and io~3 M, about 70 %. In general the inhibitors are efficacious within orders of magnitude which are similar in vivo and in vitro. Atractyloside is rather more efficacious in vitro than in vivo, and atebrine rather less. It is easier to achieve total inhibitions of the active transport of sugars in vitro than of intestinal absorption from isotonic solutions in vivo.