Serum protein titration by reference to to the normal mean

Abstract

It is proposed the employ of a pool of sera of normal subjects as a standard for the estimation of serum proteins. 7.0 g. per cent is adopted as average normal value. The range of probable error when using a pool of 9 sera is less than 4 per cent (0.3 g. per cent), i. e., not greater than that of most usual methods. By this procedure, the known divergences among different methods will be avoided.

Among the colorimetric methods it is prefered that of the biuret reaction with the author’s “one piece” reagent. Beer law desviation in visual colorimetry is corrected by the two standards colorimetry.

Albumin and globulin, and the a» and 7 fractions of the later may be easily separed with sodium sulfite at 26.8, 19, and 15 per cent.

Detailed techniques are described for the estimation of total serum protein and its fractions either by colorimetry or photometry.