Subcellular study of ethanol action on myocardium

Abstract

The effect of ethanol on calcium binding activity in plasma membrane from dog cardiac muscle is described.The binding activity to sarcolemma was measured by the centrifugation method.The ethanol effect on the activity was studied by measuring the amount of bound calcium when the preparation was incubated in an ethanol containing solution at a desired concentration.Results showed that membrane bound calcium increased with ethanol concentration of up to 180 mM.Higher concentrations only decreased the amount of bound calcium.