Phlorhizin inhibition in the absorption of monosaccharides by the cat intestine

Abstract

Succesive absorption series have been carried out, in order to study the selectivity of the different monosaccharides andtheir respective inhibitions by phlorizin M/1000.

Solz and Ponz’s (7) succesive absorption technique was used on cats of 2 to 5 K of weight, anesthethized subcutaneously with Sommi-Lefa.

The volume of isotonic solution of sugar to be absorbed was 15 mi, the repletíon pressure 15 cm of water and the experi7ments lasted 30 minutes.

Succesive and alternating absorptions of the monosacchari­ des galactose, glucose, fructose, xylose and arabinose, presen- ted the following ratcs of absorptions in the cat’s intestine, in micromol per cm d-galactose 46, d-glucose 41, d-fructose 23, d-xylose 23 and d-arabinose 1,1.

Against phlorizin M/1000, the inhibitions are the following: galactose 83. 6 %, glucose 75. 1 %, fructose 21. 7 % and xylose 59. 4 %. Consequently there is no direct proportion between the rate of absorption and the phlorizinie inhibition, as although galactose is inhibited in a máximum way, being it thc sugar which is absorbed faster, xylose of qual rate as fructose, is inhibited notably less than this last one.

The claimed increase in the intestinal absorption of fructose by action of phlorizin, cited by Bogdanove (11) and Csary (12)and which we could not verify in a previous work done on the rat’s intestine, on which we found a phlorizin inhibiting effect of 21 % (6) and we do not find it now either in the cat’s intes­ tine, inasmuch as similarly to what was found on the rat, we find a 23 % inhibition on the absorption of fructose on the cat.

The decreased. absorption of d-xylose as compared with d-glucose against those i'ndicated by Davidson (1) is clear.