Lipase activity and fat mobilization

Abstract

Using the method of RONA and Lasnitzki and the Warburg technique, the lypolysis can be studied simultaneously with the esters of fatty acids in the blood, liver and adiposo tissue. It has been possible to follow the mobility of fat and its reesterification in the liver and adiposo tissue, simulta­neously with the rcesterification of the blood as real transporter. Mobility speed has been measured by the falling indication of the descent in the straight line of the graph, esters of fatty acid, and time in hours. Recovery speed has been ascertained by the exponent increase in the figures, depending on the straight line in the same experimental conditions, using dogs that liad been fasting for 72 hours and who were then fed with food rich in carbohydrates, the final determination being carried out on an animal that was only fed again after 120 hours. The lipolytic activity is expressed in micronioles of butiric acid, freed from substratum tributirine for an hour, and per milligran of protein.