Bichromogenic substrates of rapid hydrolysis for phosphatases

Abstract

A comparativo study is made with sodium phenolphtalein phosphate on the enzymatic hydrolysis of the sodium salts of the phosphoric esters pf aurine and benzaurine, as well as of that of the phosphoric esters of 4,4’-hydroxy-azobenzene-sulphonic acid, of 4,4’-amidosulphonylbenzene-azo-phenol, of 3,4’-tolil-azo-phenol, of 4’- phenyl-azo-phenol, and of that of the isomeric esters (2, 3 and 4), 4’-cárboxyl-azobenzene-phosphoric, these latter comparatively among cach other and, excepting phenolphtaleinic es.ter, all are described for the first time.
There are exposed some practical applications of same referring to the antagonistic action exercised by para-aminobenzoic acid on amido-sulphonylbenzene-azo-phenol formed in the enzymatic hydrolisis of ester 4,4’-amidosulphonylbenzene-azo-phenylphosphoric and to the hydrolytic conduct of the isomeric esters referred to.