Induction of ribonucleases in fungi. I. Enzyme yield as a function of culture conditions

Abstract

The yields of inductive RNA in the culture of Pleospora fungi in liquid medium composed by buffered RNA, are studied in function of the concentration of RNA, of the buffer, kind of culture medium, kind of buffer, ionic strength and duration of culture. The best conditions of yield corresponding to a concentration of 0,75% RNA in citrate buffer pH 6,5 and ionic strength 0,0125. The yield obtained with phosphate buffer is very inferior to the yield obtained with citrate buffer of the same pH and concentration. The mycelium growth in funcion to the duration of culture is also studied. On the other hand, determinations of ribonuclease activity were carried out, and it appears that the results of those show a relationship between growth and absolute activity. The relative activity is, however, in inverse relation to the growth.