Induction of phosphomonoesterases in microorganisms. I. Enzymatic induction depending on the condition of the culture

Abstract

In this work are studied the conditions in which fungi (Fusariurn solani) developed itself and inducted phosphomonoes­ terases in liquid medium composed by buffered RNA. Thoroughly are studied the concentration of RNA, kind of cultu­re, kind of buffer, pH of the buffer, ionic stregth and duration of culture.

The best conditions corresponding, to 0.75 % RNA in citrate buffer pH 4.9 and ionic strength 0.0125 for the myeelium growth, and the same conditions, except pH which is 4.0 for the induction of phos­phomonoesterases. In phosphate buffer there is a greater induction, though the growth is very lower.

The enzymatic absolute activity is proportional to myeelium growth.