Induction of phosphomonoesterases in microorganisms. I. Enzymatic induction depending on the condition of the culture

J.M. Ventura
C.M. Cuchillo
E. Concustell
V. Villar-Palasí
39

Abstract




In this work are studied the conditions in which fungi (Fusariurn solani) developed itself and inducted phosphomonoes­ terases in liquid medium composed by buffered RNA. Thoroughly are studied the concentration of RNA, kind of cultu­re, kind of buffer, pH of the buffer, ionic stregth and duration of culture.


The best conditions corresponding, to 0.75 % RNA in citrate buffer pH 4.9 and ionic strength 0.0125 for the myeelium growth, and the same conditions, except pH which is 4.0 for the induction of phos­phomonoesterases. In phosphate buffer there is a greater induction, though the growth is very lower.


The enzymatic absolute activity is proportional to myeelium growth.




Keywords:
Enzyme Induction, Culture Media, Fungi/enzymology, Phosphoric Monoester Hydrolases/biosynthesis, RNA, Time Factors

Authors

J.M. Ventura
C.M. Cuchillo
E. Concustell
V. Villar-Palasí


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