Induction of ribonuclease in fungi. 3. Kinetics of the enzyme reaction

Abstract

The most important aspeets of the kinetics of the breakdown of yeast RNA by the action of the Pleospora-induced ribonuclease have been studied. Factors involved in this study were enzyme concentration, substrate concentration, time and pH.

Through the reaction, first-order ki­netics are observed, at least during the early stages of the reaction; the optimal pH has a value of 7.5 and the Michaelis-Menten constant has a valué of 9.8 x ro-3 gr/100 mi.

Phosphate ions act as weak inhibitors of the Pleospora adaptative ribonu­clease.