Induction of ribonuclease in fungi. 3. Kinetics of the enzyme reaction

C.M. Cuchillo
J.M. Ventura
E. Concustell
V. Villar-Palasí
38

Abstract




The most important aspeets of the kinetics of the breakdown of yeast RNA by the action of the Pleospora-induced ribonuclease have been studied. Factors involved in this study were enzyme concentration, substrate concentration, time and pH.


Through the reaction, first-order ki­netics are observed, at least during the early stages of the reaction; the optimal pH has a value of 7.5 and the Michaelis-Menten constant has a valué of 9.8 x ro-3 gr/100 mi.


Phosphate ions act as weak inhibitors of the Pleospora adaptative ribonu­clease.




Keywords:
Ascomycota/enzymology, Hydrogen-Ion Concentration, Kinetics, RNA/metabolism, Ribonucleases/biosynthesis

Authors

C.M. Cuchillo
J.M. Ventura
E. Concustell
V. Villar-Palasí


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