Tests on differential staining of cellular structures and tissues with tetrabromophenolsulfophthalein

Abstract

The use of tetrabromophenolsuphophthalein is proposed as an histological differential stainer of cells and cellular structures. The reagent must be used dissolved in a buffer solution of 3,8 pH, obtained with the mixture of disodium phosphate 0,2 molar and citric acid 0,1 molar, although it furnishes appreciable results in a methanolic solution, too.

Those cellular components whose isoelectric point is below 3, are turned yellow by the reagent, and when above 4,6, blue.

The colours, However, must not be put on a par with those obtained by means of the reagent in different pure buffer Solutions, owing to the great influence exerted on results by the reaction of the cellular proteins with the colouring stuff.