Cellular localization, half-life, and secretion of peptide YY

F. Lluis
M. Fujimura
G. Gómez
J.A. Salvá
G.H.Jr Greeley
J.C. Thompson
57

Abstract

Tissue and plasma concentration of peptide YY (PYY) were measured by means of a radioimmunoassay (RIA) developed in our laboratory, using a specific PYY antiserum generated in New Zealand white rabbits against synthetic PYY, and dextran-coated charcoal to terminate the assay.Cellular localization of PYY was studied immunohistochemically using the peroxidase-antiperoxidase (PAP) technique.The highest tissue concentration of PYY was found in the mucosa of the terminal ileum and colon.PYY-containing secretory granules were primarily found in the basal pole of open-type endocrine cells.Basal plasma concentration of PYY was 70 +/- 9 pg/ml and rose to 357 +/- 30 pg/ml during the IV administration of PYY at 400 pmol/kg-h.A significant correlation was found (r = 0.94, p less than 0.05) between dose of PYY (12.5, 25, 50, 100, 200, 400 pmol/kg-h, IV) and plasma concentration of PYY.The calculated half-life of PYY in plasma was 8.3 +/- 1.9 minutes.Plasma concentration of PYY during the intraduodenal administration of sodium oleate (150 +/- 20 pg/ml) or long-chain triglyceride (187 +/- 37 pg/ml) was similar to plasma concentration of PYY obtained during the IV administration of PYY at 100 pmol/kg-h.Plasma concentration of PYY raised (126 +/- 10 pg/ml) after the administration of bombesin (400 pmol/kg-h, IV).Bile enhanced release of PYY.The present study suggests a hormonal role for PYY.

Keywords:
Amino Acids/pharmacology, Animals, Bile/physiology, Bombesin/pharmacology, Digestive System/analysis, Dogs, Female, Half-Life, Hydrochloric Acid/pharmacology, Immunoenzyme Techniques, Male, Oleic Acid, Oleic Acids/pharmacology, Peptide YY, Peptides/analysis/metabolism/pharmacokinetics, Secretory Rate/drug effects, Triglycerides/pharmacology, Gastrointestinal Endocrinology, Radioimmunoassay, Immunohistochemistry, Half-life, Release

Authors

F. Lluis
M. Fujimura
G. Gómez
J.A. Salvá
G.H.Jr Greeley
J.C. Thompson


Metrics

Search GoogleScholar



Downloads

Download data is not yet available.

Section

Articles

Most read articles by the same author(s)